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Blue Veil
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In the gel image, appears as a blue haze migrating downward like a
veil
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Dye Blobs
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Bright fluorescence
superimposed on sequence, caused by the inefficient removal of unincorporated
dye terminators post-PCR. Observed in both the gel image and the
trace file view as large wide peaks.
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G-Stop
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In the trace file view, observed as an abrupt termination of
signal following a run of Gís.
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Mobility Error
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Wrong mobility file applied, causing base peaks to appear shifted
particularly at the start of the sequence.
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Multiple Priming Sites
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Observed as multiple sequences/peaks superimposed, often due to extension
products being generated from multiple priming sites
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Red Rain
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From the gel image, appears as red streaks migrating down from the
top of the screen
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Slippage
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Polymerase slippage during PCR amplification and/or cycle
sequencing due to a homopolymeric region in the template. Electropherogram
may show a stutter or sawtooth pattern
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Spike
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Large sharp peaks in data, may be one-color or four-color.
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Trailing Signal
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Signal intensity decreases, appearing as gradually decreasing peak
heights
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Waterfall
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This refers to the raw data baseline. A raised baseline in all colors is observed at the start, which
then drops after a given amount of time in a waterfall pattern. There may be a signal dropout visible
in the electropherogram at this point.
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